2-(6-Nitro-1-oxy-benzotriazol-3-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (NBTU): A new coupling regent in oligonucleotide synthesis.

Tushar K Srivastava, Seturam B Katti

Abstract


A new coupling reagent 2 - (6 - nitro - l - oxy - benzotriazol - 3 - yl ) - I, I, 3, 3 - tetramethyluronium hexafluorophosphate (NBTU) has been developed for use in solution and solid-phase methods of oligonucleotide synthesis. The reagent is also appli- cable to anchor the leader nucleoside on to the Icaa-CPG.(5a-e).[PhSeSePhr are found to effect stereoselective oxyselenylation of I,n- dioletins (I) leading to a novel methodology to the synthesis of a,a' -trans-dialkyl cyclic ethers (8). Keywords: Photosensitized electron transfer (PET), episelenonium radical cation, oxyselenylation, a,a' -trans-dialkyl cyclic ethers.been subjected to some kind of chemical or biological examination; thus vast majority of natural wealth remains unexplored. Studies on marine natural products initiated only recently have yielded molecules with exotic molecular architecture and diverse bioactivity. Well-directed research on natural products will enrich our bioresource base. In the current millennium, India is in a pre-eminent position to contribute towards natural product research with the utilization of its rich knowledge base and biodiversity.

Keywords


Coupling reagent; oligonucleotide synthesis; H-phosphonate; phosphotriester.ylide.biocatalytic synthesis; chemical ecology; bioremedia- tion.

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